Journal of Current Research in Food Science
2024, Vol. 5, Issue 2, Part C
Unravelling the secrets of fenugreek through integrated approaches
Author(s): Layla Al-Sabah, Ahmed Jasim Al-Mukhtar and Omar Rami Al-Fadhli
Abstract: Fenugreek (Trigonella foenum-graecum L.) has long been valued for its medicinal and nutritional properties. This study explores the bioactive potential of fenugreek through an integrated approach combining extraction techniques, bioactivity assays, and omics-based analyses. Hydroalcoholic solvents, particularly 50% ethanol, were found to yield the highest concentration of bioactive compounds, including diosgenin, trigonelline, and galactomannan. The antioxidant capacity of fenugreek was evaluated using DPPH and ABTS assays, with methanol and 50% ethanol extracts exhibiting the strongest radical-scavenging activity. Antidiabetic and hypocholesterolemic effects were assessed through glucose uptake assays and α-amylase inhibition, showing significant improvements with methanol and 50% ethanol extracts. Antibacterial activity was also demonstrated against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa, suggesting its potential as a natural antimicrobial agent. Additionally, transcriptomic analysis revealed enhanced expression of key genes involved in saponin and flavonoid biosynthesis. These findings highlight the therapeutic potential of fenugreek and support its use in functional foods and nutraceuticals. Future research should focus on clinical validation and optimization of fenugreek's bioactive potential for human health applications.
Pages: 183-188 | Views: 332 | Downloads: 107
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How to cite this article:
Layla Al-Sabah, Ahmed Jasim Al-Mukhtar, Omar Rami Al-Fadhli. Unravelling the secrets of fenugreek through integrated approaches. J Curr Res Food Sci 2024;5(2):183-188.
